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pcas guide puro crispri scramble vector  (OriGene)


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    Structured Review

    OriGene pcas guide puro crispri scramble vector
    Pcas Guide Puro Crispri Scramble Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcas+guide+vector/pCas-Guide-Puro-CRISPRi-Scramble/pmc13219498-365-1-3
    Average 94 stars, based on 3 article reviews
    pcas guide puro crispri scramble vector - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Reducing CXCR4 expression and/or function to enhance engraftment of hematopoietic stem cells
    Article Snippet: .. In one example for plasmid delivery to an HSC, the pCas-Guide vector from Origene can be used. .. In one example, for example for viral delivery to an HSC, the pLenti-Cas Guide vector from Origene (Rockville, Md.) can be used.

    Article Title: Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
    Article Snippet: .. Generation of AD-MSC AhR-KO cell clones The AhR gene was knocked out in AD-MSCs using the pCas-Guide CRISPR vector carrying aryl hydrocarbon receptor gRNA (target sequence: CCTACGCCAGTCGCAAGCGG in exon 1 of the AhR gene, NCBI Reference Sequence: NM_001621), a donor vector containing the left and right homologous arms and GFP-Puro functional cassette, and a scramble sequence in the pCas-Guide vector (Origene, USA). ..

    Article Title: The ER Thioredoxin-Related Transmembrane Protein TMX2 Controls Redox-Mediated Tethering of ER-Mitochondria Contacts (ERMCS)
    Article Snippet: U251 TMX2 KO cells were generated with the TMX2 Human Gene Knockout Kit (OriGene, KN400032). .. The pCas-Guide vector (OriGene, GE100003) with a scrambled sequence was used as a control. .. Briefly, U251 MG cells were co-transfected with a pCas-Guide vector (target sequence: CCAACCTTACTACTACCTTCTGT) and a linear donor vector (containing EF1a-GFP-P1A-Puro) using Lipofectamine 3000.

    Article Title: Transcriptomic Investigation in CRISPR/Cas9-Mediated GRIK1-, GRIK2-, and GRIK4-Gene-Knockout Human Neuroblastoma Cells
    Article Snippet: .. In brief, the pCas-Guide vector carrying the guide RNA (gRNA) guide sequence was generated using the method described in the manufacturer’s protocols (Origene, Rockville, MD, USA). .. The SH-SY5Y cells (Sigma catalog no. 94030304) were transfected with a pCas-Guide vector carrying gRNA using a Neon electroporation transfection system (Invitrogen, Carlsbad, CA, USA).

    Article Title: Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC -Knockout HEK293 Cells
    Article Snippet: .. A pCas-Guide vector carrying the gRNA guide sequence (CCATCTGTGCGCAGGTGCTC) was generated using the protocol of the manufacturer (Origene, Rockville, MD, USA). .. Briefly, two oligos were annealed using the following conditions: 94 °C for 4 min, 75 °C for 5 min, 65 °C for 15 min, and 25 °C for 20 min. After annealing, double-stranded oligos were ligated into the precut pCas-Guide vector, and the ligation mixture was transformed into DH5α-competent cells.

    Article Title: Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes
    Article Snippet: .. This plasmid was co-transfected with the pCas-Guide vector following the instructions from Origene. ..

    Article Title: Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes.
    Article Snippet: .. This plasmid was cotransfected with the pCas-Guide vector following the instructions from Origene. ..

    Article Title: Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions
    Article Snippet: .. Deletion of ZAK in HEK293T cells was achieved by CRISPR using a pCas-Guide vector (Origene, #GE100002) and linear donor DNA containing LoxP-EF1A-tGFP-P2A-Puro-LoxP (Origene, #KN402432D). ..

    Clone Assay:

    Article Title: Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
    Article Snippet: .. Generation of AD-MSC AhR-KO cell clones The AhR gene was knocked out in AD-MSCs using the pCas-Guide CRISPR vector carrying aryl hydrocarbon receptor gRNA (target sequence: CCTACGCCAGTCGCAAGCGG in exon 1 of the AhR gene, NCBI Reference Sequence: NM_001621), a donor vector containing the left and right homologous arms and GFP-Puro functional cassette, and a scramble sequence in the pCas-Guide vector (Origene, USA). ..

    CRISPR:

    Article Title: Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
    Article Snippet: .. Generation of AD-MSC AhR-KO cell clones The AhR gene was knocked out in AD-MSCs using the pCas-Guide CRISPR vector carrying aryl hydrocarbon receptor gRNA (target sequence: CCTACGCCAGTCGCAAGCGG in exon 1 of the AhR gene, NCBI Reference Sequence: NM_001621), a donor vector containing the left and right homologous arms and GFP-Puro functional cassette, and a scramble sequence in the pCas-Guide vector (Origene, USA). ..

    Article Title: Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions
    Article Snippet: .. Deletion of ZAK in HEK293T cells was achieved by CRISPR using a pCas-Guide vector (Origene, #GE100002) and linear donor DNA containing LoxP-EF1A-tGFP-P2A-Puro-LoxP (Origene, #KN402432D). ..

    Sequencing:

    Article Title: Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
    Article Snippet: .. Generation of AD-MSC AhR-KO cell clones The AhR gene was knocked out in AD-MSCs using the pCas-Guide CRISPR vector carrying aryl hydrocarbon receptor gRNA (target sequence: CCTACGCCAGTCGCAAGCGG in exon 1 of the AhR gene, NCBI Reference Sequence: NM_001621), a donor vector containing the left and right homologous arms and GFP-Puro functional cassette, and a scramble sequence in the pCas-Guide vector (Origene, USA). ..

    Article Title: The ER Thioredoxin-Related Transmembrane Protein TMX2 Controls Redox-Mediated Tethering of ER-Mitochondria Contacts (ERMCS)
    Article Snippet: U251 TMX2 KO cells were generated with the TMX2 Human Gene Knockout Kit (OriGene, KN400032). .. The pCas-Guide vector (OriGene, GE100003) with a scrambled sequence was used as a control. .. Briefly, U251 MG cells were co-transfected with a pCas-Guide vector (target sequence: CCAACCTTACTACTACCTTCTGT) and a linear donor vector (containing EF1a-GFP-P1A-Puro) using Lipofectamine 3000.

    Article Title: Transcriptomic Investigation in CRISPR/Cas9-Mediated GRIK1-, GRIK2-, and GRIK4-Gene-Knockout Human Neuroblastoma Cells
    Article Snippet: .. In brief, the pCas-Guide vector carrying the guide RNA (gRNA) guide sequence was generated using the method described in the manufacturer’s protocols (Origene, Rockville, MD, USA). .. The SH-SY5Y cells (Sigma catalog no. 94030304) were transfected with a pCas-Guide vector carrying gRNA using a Neon electroporation transfection system (Invitrogen, Carlsbad, CA, USA).

    Article Title: Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC -Knockout HEK293 Cells
    Article Snippet: .. A pCas-Guide vector carrying the gRNA guide sequence (CCATCTGTGCGCAGGTGCTC) was generated using the protocol of the manufacturer (Origene, Rockville, MD, USA). .. Briefly, two oligos were annealed using the following conditions: 94 °C for 4 min, 75 °C for 5 min, 65 °C for 15 min, and 25 °C for 20 min. After annealing, double-stranded oligos were ligated into the precut pCas-Guide vector, and the ligation mixture was transformed into DH5α-competent cells.

    Functional Assay:

    Article Title: Knock out aryl hydrocarbon receptor gene in adipose-derived mesenchymal stem cell line (PT-5006) by CRISPR/Cas9
    Article Snippet: .. Generation of AD-MSC AhR-KO cell clones The AhR gene was knocked out in AD-MSCs using the pCas-Guide CRISPR vector carrying aryl hydrocarbon receptor gRNA (target sequence: CCTACGCCAGTCGCAAGCGG in exon 1 of the AhR gene, NCBI Reference Sequence: NM_001621), a donor vector containing the left and right homologous arms and GFP-Puro functional cassette, and a scramble sequence in the pCas-Guide vector (Origene, USA). ..

    Control:

    Article Title: The ER Thioredoxin-Related Transmembrane Protein TMX2 Controls Redox-Mediated Tethering of ER-Mitochondria Contacts (ERMCS)
    Article Snippet: U251 TMX2 KO cells were generated with the TMX2 Human Gene Knockout Kit (OriGene, KN400032). .. The pCas-Guide vector (OriGene, GE100003) with a scrambled sequence was used as a control. .. Briefly, U251 MG cells were co-transfected with a pCas-Guide vector (target sequence: CCAACCTTACTACTACCTTCTGT) and a linear donor vector (containing EF1a-GFP-P1A-Puro) using Lipofectamine 3000.

    Generated:

    Article Title: Transcriptomic Investigation in CRISPR/Cas9-Mediated GRIK1-, GRIK2-, and GRIK4-Gene-Knockout Human Neuroblastoma Cells
    Article Snippet: .. In brief, the pCas-Guide vector carrying the guide RNA (gRNA) guide sequence was generated using the method described in the manufacturer’s protocols (Origene, Rockville, MD, USA). .. The SH-SY5Y cells (Sigma catalog no. 94030304) were transfected with a pCas-Guide vector carrying gRNA using a Neon electroporation transfection system (Invitrogen, Carlsbad, CA, USA).

    Article Title: Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC -Knockout HEK293 Cells
    Article Snippet: .. A pCas-Guide vector carrying the gRNA guide sequence (CCATCTGTGCGCAGGTGCTC) was generated using the protocol of the manufacturer (Origene, Rockville, MD, USA). .. Briefly, two oligos were annealed using the following conditions: 94 °C for 4 min, 75 °C for 5 min, 65 °C for 15 min, and 25 °C for 20 min. After annealing, double-stranded oligos were ligated into the precut pCas-Guide vector, and the ligation mixture was transformed into DH5α-competent cells.



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